FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRa-Rep2_S20_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRa-Rep2_S20_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8270595
Sequences flagged as poor quality0
Sequence length15-23
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAT1450.035.9156617
AGACGAT900.034.2898517
TTTCGAT1050.033.0652117
AGCCTAC13050.029.1168117
GTCGTTG602.8863724E-828.9320617
ACCATCT22500.028.88919617
CCAACTT16200.028.81317
TACGTTC2400.028.12839117
AGTCGAT2750.028.0553317
GGCCTAC16650.027.68673517
CATCGAT1000.027.00325417
AACCTTC27100.026.40539717
TACCTTC16250.026.23173317
AACCTAC8050.026.11672217
GTCCATC60250.025.86678117
GACCTTC32450.025.85607717
GACCTAC11350.025.49079917
ACCCATC55650.025.16283217
CACCTTC44650.024.92541717
CGCCATC48450.024.88137217
Produced by FastQC (version 0.11.5)