FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRa-Rep2_S20_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRa-Rep2_S20_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8589409
Sequences flagged as poor quality0
Sequence length15-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTC12300.032.11214417
TGTCGAT1900.031.378717
GACGTTC11250.031.13464217
TACGTTC6200.030.3506717
AGACGAT1450.029.55278417
TTCGTTC10350.028.08166517
ATCGCAT405.6645236E-527.94665517
GCCGTTC20700.027.81164717
AGCCTAC11850.027.5142917
ATCGTTC6700.027.25146517
AGCGTTC13600.026.43972617
GACCTAC12500.026.38164117
CTCGTTC11450.026.03473317
ACCTTCT23050.025.94613517
AACCTTC37200.025.69289417
ACCCTTC42500.025.55749117
GGCGTTC20700.025.38150417
ACCGTTC10500.025.37378917
GTCGTTG751.2763849E-824.84147317
GCCCTAC18100.024.8071617
Produced by FastQC (version 0.11.5)