FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRa-Rep2_S20_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRa-Rep2_S20_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8526129
Sequences flagged as poor quality0
Sequence length17-23
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAT1250.039.31909617
ACCATCT16550.030.47191217
AGACGAT1000.029.91670417
ACCCATC44800.029.57327317
CCAACTT11250.029.25188817
GTCCATC45250.029.23194917
CGCCAAC151800.028.66102417
AACCATC40850.028.56184617
CGCCATC38400.028.49209817
GACCATC45550.028.3357217
TACCATC24050.027.72204217
CCCCAAC232600.027.1109817
GACCTAC8100.026.90920417
TACCTTC10900.026.85837617
AGCCATC48250.026.70580717
TCCCAAC241900.026.63404717
TTCCATC36550.026.6017217
GCCCAAC259500.026.28519617
CCCCATC62800.026.2349617
ATCCATC33000.026.2256817
Produced by FastQC (version 0.11.5)