FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRa-Rep2_S20_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRa-Rep2_S20_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8267640
Sequences flagged as poor quality0
Sequence length16-23
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAT3100.035.5443817
AGCCTAC17750.032.0189317
GGCCTAC27650.031.04171217
AGACGAT1750.030.92984617
GACGTTC11900.030.53997817
AACCTAC13300.030.08683617
GCCCTAC25200.029.99397517
TACCTAC10800.029.89169317
GACCTAC21150.029.88788617
AACCTTC40250.029.8252117
CCCCTAC25950.029.72304717
GTCGTTC13000.029.44283517
CTCGTAC2700.029.35471717
TTTCGAT1650.029.28962717
TCCGTAC3050.029.15518417
TACGTTC4850.029.09637817
GGCGTAC4700.028.79108417
ACCCTTC44650.028.79108217
ATCGTTC6200.028.68493817
TACCTTC21300.028.67908717
Produced by FastQC (version 0.11.5)