FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRa-Rep1_S17_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRa-Rep1_S17_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7656437
Sequences flagged as poor quality0
Sequence length17-23
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAT1150.035.2829417
TGTCGAT1300.031.2118317
GTCGTTG1000.030.91457617
GTCGTTC5650.029.06790717
TTTCGAT1200.028.98241217
ACCATCT19500.028.5365317
AGCCTAC9650.027.43067717
TACGTTC2550.027.27756717
AACCTAC7900.027.14808317
AACCTTC26750.027.01413717
GGCCTAC14750.026.98475617
TACCTTC13700.026.6553617
ACCCTTC28800.026.43303517
CGCCAAC110500.026.2983717
CCAACTT15900.026.00518617
CGCCATC43150.025.70244217
GACCTTC28300.025.67111217
GTCCATC55400.025.59938817
ACCCATC50150.025.19707317
AACCATC44600.025.04011217
Produced by FastQC (version 0.11.5)