FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRa-Rep1_S17_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRa-Rep1_S17_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7937031
Sequences flagged as poor quality0
Sequence length16-23
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAT1000.035.38698617
GACGTTC11250.031.455117
TACGTTC5100.031.40640817
TGTCGAT1750.030.8638417
GTCGTTC12500.030.24656117
GCCGTTC20250.029.98351717
GGCCTAC14550.027.77708817
CTCGTTC10800.027.59219217
AACCTAC7600.027.44697217
AATCGAT1500.027.31627317
ATCGTTC5850.027.06157317
TTCGTTC9700.026.88105617
ACCTTCT23850.026.39479617
AGCGTTC12100.026.320917
AGCCTAC10900.026.31383917
ACCCTTC39500.026.21607617
GGCGTTC19500.025.21502117
CGCCAAC104150.025.05352617
AACGTTC9050.024.69577417
GACCTTC42400.024.33514217
Produced by FastQC (version 0.11.5)