FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRa-Rep1_S17_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRa-Rep1_S17_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7874480
Sequences flagged as poor quality0
Sequence length16-23
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAT1250.037.42778417
AGACGAT508.020652E-834.02525317
GTCCATC43250.030.04252817
ACCATCT14600.030.00514817
ACCCATC39150.029.33211517
CGCCATC35350.028.514817
CGCCAAC136600.028.38032517
TACGTTC1050.028.35437817
GACCATC42300.028.25383217
AACCTTC18900.028.12934517
AGCCATC44650.028.05273417
AACCATC34750.027.84440817
TTTCGAT1150.027.7379817
TCCCATC51350.027.66415417
CCCCATC58650.027.15783717
CCCCAAC214200.027.0041717
CCAACTT11950.026.87140817
AGCCTAC8800.026.82388917
TCCCAAC224700.026.34799617
ATCCATC32650.026.1832917
Produced by FastQC (version 0.11.5)