FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRa-Rep1_S17_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRa-Rep1_S17_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7654253
Sequences flagged as poor quality0
Sequence length15-23
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTC9300.033.21031617
CTCGTAC3150.032.68316717
AGACGAT1800.032.37483617
TGTCGAT2300.032.09331517
GTCGTAC2000.032.05108617
AGCCTAC17700.031.82610917
AGCGTAC3250.031.67753217
GGCCTAC23550.031.59124217
AACCTAC13150.031.16847417
GACCTAC17700.031.16763717
TCCGTAC3450.030.96723417
TACCTAC9300.030.28613717
CCCCTAC27150.029.76338617
GACGTTC9150.029.50886517
GCCCTAC24800.029.29400417
GTCGTTG602.6946509E-829.13735217
CATCGAT1800.029.13735217
AACCTTC36050.029.0969417
TACCTTC20100.028.70246717
TACGTTC4350.028.57916517
Produced by FastQC (version 0.11.5)