FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRKO-T0_S3_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRKO-T0_S3_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7100741
Sequences flagged as poor quality0
Sequence length15-23
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAT207.746799E-444.12790317
ATCGAAC254.3689026E-544.12790317
CGTCGAT302.4789533E-644.127917
TACGTTC1650.040.11627617
CGCGTTC404.0343912E-738.61191617
ATCGTTC2150.037.9705217
CCAACTT52800.036.8150417
GCTCGAT800.035.8539217
GTCGTTC2750.035.30232217
CCATCTT24000.035.02652417
GGACGAT1000.033.0959317
CCAAGTT41150.032.54633717
CCAACAT58500.032.32274617
ACTCGTT551.340195E-732.0930217
CTCGTTC3850.031.51993217
AGACGAT1150.030.69767217
CACGTTC5500.030.08720817
AGGCGAT1950.029.418617
GTTCGAT300.00566528929.418617
TACCGAT300.00566528929.418617
Produced by FastQC (version 0.11.5)