FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRKO-T0_S3_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRKO-T0_S3_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7360119
Sequences flagged as poor quality0
Sequence length18-23
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTC1200.042.57580617
CGTCGAT209.2403183E-442.575817
TACGTTC4500.042.10273717
GTCGTTC6900.041.033217
GACGTAC700.039.5346717
GGCGTTC8300.039.2415517
CCATCTT52850.039.07145717
ATCGTTC4750.038.9904717
TACGTAC800.037.25382617
CACGTTC9300.036.8532517
GCCGTTC11700.036.57152617
GACGCTT2150.035.64485517
GTCGCTT2750.035.60885217
TTCGTTC8050.035.4357617
AGCGTAC1150.035.17131817
CTCGTTC8200.035.04715317
AGCGTTC7850.034.98266617
TCCGTTC7750.034.8846917
AACGTTC7250.034.35426717
ATCGTAC250.002765406234.06064217
Produced by FastQC (version 0.11.5)