FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRKO-T0_S3_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRKO-T0_S3_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7339919
Sequences flagged as poor quality0
Sequence length18-23
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTC800.046.60153617
GAGCGAT458.016286E-944.18515817
TTTCGAT700.042.60711717
TACGTTC700.042.60711717
CGCGTTC453.9933911E-738.66201417
CCAACTT32150.038.11227417
CCAAGTT34100.037.02612717
GGACGAT554.7055437E-836.15149317
CGCCGAT351.5725991E-435.5059317
TACCGAT509.112846E-734.7958117
AACAACG3650.034.7277217
GTCGTTC1850.033.58669317
GATCGTT601.01126716E-733.1388717
TGGCGAT755.875336E-1033.13886617
CCAACAT48950.032.69882217
CTACGAT551.9188847E-631.63255717
GTACGTT403.444311E-431.0676917
ACCATCT26100.030.66297317
AACAAGG37300.029.85163317
TATCGTT503.6346955E-529.82498217
Produced by FastQC (version 0.11.5)