FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRKO-T0_S3_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRKO-T0_S3_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7136532
Sequences flagged as poor quality0
Sequence length15-23
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTTC3800.043.20186217
ATCGTTC4300.042.88531517
TTCGTAC1800.042.47854217
CGCGTTC1400.041.76461817
GTCGTTC6850.041.69426717
TACGTAC1500.040.4795517
GACGTAC1850.040.1148717
GGCGTTC7800.038.92264617
CGTCGAT356.273025E-638.55195217
CGCCGAT356.273025E-638.55195217
AGCGTAC1950.038.05769717
CGGCGAT301.1437113E-437.48106417
AGCGTTC6650.037.19925717
CCAAGTT57850.036.6971117
CTCGTTC6450.036.60941317
TGCGTTC7300.036.35149817
ATCGTAC751.8189894E-1235.98182317
TCGCGAT250.002112824735.98182317
CACGTTC8800.035.7773817
ACCCTAC11650.035.5185417
Produced by FastQC (version 0.11.5)