FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRKO-Rep2_S9_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRKO-Rep2_S9_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5838315
Sequences flagged as poor quality0
Sequence length16-23
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAT351.1406519E-745.35127617
TACGTTC1500.040.81614717
GTCGTTC2550.040.01583517
ATCGTTC1400.038.87252417
AGCGTTC3350.038.58242817
CGCGTTC301.08922766E-437.79273217
ACGCGTT301.08922766E-437.79273217
CCAACTT41300.037.71952417
TCCGTTC2550.036.4588717
GGCGTTC2000.036.28102517
TGTCGAT1400.035.6331517
CCATCTT20100.035.5364517
CACGTTC3200.035.43068717
CCAAGTT33450.033.55587817
ACCCTAC5850.032.17227617
AGTCGAT1350.031.91386217
GGACGAT501.8659157E-631.74589517
ATACGAT501.8659157E-631.74589517
TGGCGAT901.0913936E-1130.23418617
ATCGTTG300.004956146230.23418617
Produced by FastQC (version 0.11.5)