FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRKO-Rep2_S9_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRKO-Rep2_S9_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6060233
Sequences flagged as poor quality0
Sequence length16-23
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC351.7402635E-742.97835517
ATCGTTC3750.042.40530817
TACGTTC3800.040.15083317
AGACGAT750.040.1131317
CCATCTT45200.039.1274217
CGCGTTC1050.038.88517817
GTCGTTC6900.037.99535417
GGCGTTC7550.037.85510317
GAGCGAT404.9612026E-737.6060617
TCCGTTC6500.037.35810517
GACGTAC750.037.24790617
AGCGTTC6100.036.98956717
CACGTTC8100.036.08059317
CTCGTTC5800.035.93879317
TGCGTTC7200.035.51683417
TTCGTAC1100.035.1641117
GCCGCTT2750.035.1641117
TACGTAC554.312824E-935.1641117
GCCGTTC10350.035.08860817
TTCGTTC5700.035.06128717
Produced by FastQC (version 0.11.5)