FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRKO-Rep2_S9_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRKO-Rep2_S9_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6052104
Sequences flagged as poor quality0
Sequence length16-23
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAT309.911128E-750.40815417
CGTCGAT150.00816524450.40815417
TACGTTC650.046.53059817
ACTCGTT551.8189894E-1145.82559217
ATCGTTC700.043.20698517
GAGCGAT352.865674E-643.20698517
TATCGTT352.865674E-643.20698517
CGCGTTC250.001206764740.3265217
AACAACG4450.039.6468617
CCAACTT28150.038.58954217
GAACGTT850.038.5474117
AATCGAT950.037.14284517
CCAAGTT27700.036.30478717
GTTCGAT351.4483822E-436.0058217
GATCGTT508.167699E-735.28570617
GTCGTTC1500.035.28570617
TTTCGAT451.6076707E-533.60543417
CCAACAT42450.033.0710717
TTCGTTC1850.032.69717817
GGACGAT709.569703E-932.4052417
Produced by FastQC (version 0.11.5)