FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRKO-Rep2_S9_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRKO-Rep2_S9_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5867512
Sequences flagged as poor quality0
Sequence length15-23
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAC1050.045.55654517
CGCCGAT206.621271E-445.5565417
GACGTAC1050.043.38718417
TTCGTAC950.043.1588317
GTCGTTC6950.042.60684217
ATCGTTC3200.041.99743717
TACGTTC3800.041.95997217
CGCGTTC1250.041.9120217
GGCGTTC6700.040.79690617
AACGTTG451.7313141E-840.4947117
ATCGTAC850.040.19695317
AGCGTTC5950.039.43129317
GAGCGAT355.7449324E-639.04846617
ATCGAAC355.7449324E-639.04846617
AACGTTC4800.038.4383317
CCAAGTT48450.038.2693817
GACGATC601.364242E-1037.96378717
TCCGTTC5400.037.54196517
GCCGTTC9000.037.2045117
CACGTTC7150.036.9549617
Produced by FastQC (version 0.11.5)