FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRKO-Rep1_S6_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRKO-Rep1_S6_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7245278
Sequences flagged as poor quality0
Sequence length17-23
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT254.1095296E-544.5877817
TACGTTC1650.043.23663717
ATCGTTC2400.040.87213517
GTCGTTC2500.038.34549317
TACCGAT301.2037888E-437.15648717
CCAACTT48400.036.25060317
CGCGTTC800.036.22757317
CCATCTT25800.036.03314617
CGTCGAT250.002204963535.67022317
GGCGTTC3450.035.5409917
TGTCGAT1950.033.15501817
CCAAGTT44100.032.65726517
GTGCGAT551.2236342E-732.4274817
CCAACAT59700.031.14423217
TCACGAT651.5366822E-830.86846417
ACTCGAT651.5366822E-830.86846417
ACCCTAC7250.030.7501917
TGGCGAT801.1277734E-1030.654117
TTTCGAT950.030.50743117
CGCCGAT300.005385370529.7251917
Produced by FastQC (version 0.11.5)