FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRKO-Rep1_S6_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRKO-Rep1_S6_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7504712
Sequences flagged as poor quality0
Sequence length18-23
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGAT351.7302955E-743.01038717
CGTCGAT302.957473E-643.01038717
TACGTTC4900.040.81598317
GTCGTTC9250.040.68550517
ATCGTTC5800.040.41493617
AGCGTAC501.6862032E-938.7093517
CCATCTT54250.038.6102517
GGCGTTC8400.038.40213417
GACGTAC850.037.95034417
CGCGTTC1650.037.7970117
GTCGCTT1700.036.68533317
AGCGTTC9750.036.6139717
CTCGTTC7350.036.57346317
CACGTTC9100.036.3934117
TCCGTTC8100.036.10748717
TTCGTTC8150.035.35823417
GACGTTC7900.034.8438617
GCCGTTC12350.034.8262317
TGCGTTC9150.034.7843617
AACGTTC6850.033.59205617
Produced by FastQC (version 0.11.5)