FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRKO-Rep1_S6_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRKO-Rep1_S6_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7493867
Sequences flagged as poor quality0
Sequence length17-23
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAT203.9533543E-450.57994517
GGTCGTT1150.043.9825617
ATCGTTC1050.043.35423717
ACGCGTT352.7996593E-643.35423317
TACGTTC750.040.46395517
CCAACTT33350.040.11512817
GGACGAT602.053639E-937.9349617
GTCGTTC1500.037.0919617
GATCGTT554.0379746E-836.7854117
CCAAGTT33750.036.49249317
TAGCGTT702.3283064E-1036.1285317
GAGCGAT351.4197866E-436.1285317
AACAACG5650.034.01836817
GACGTAC300.002905005133.71996317
CCGCGAT300.002905005133.71996317
GTACGTT300.002905005133.71996317
TTTCGAT709.260475E-932.51567517
CCAACAT53950.032.48554217
AGTCGAT1900.031.94522917
TATCGTT403.1105385E-431.61246517
Produced by FastQC (version 0.11.5)