FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRKO-Rep1_S6_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRKO-Rep1_S6_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7278411
Sequences flagged as poor quality0
Sequence length17-23
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT302.0182288E-645.46600317
GACGTAC1450.043.8982117
TACGTTC4350.042.33041417
ATCGTTC4600.042.00663417
CGCGTTC1300.041.96861617
GGACGAT900.040.41422317
GTCGTTC6650.039.99640717
AGACGAT1100.039.2660917
TCCGTTC7200.039.1512817
CGTCGAT700.038.9708617
GGCGTTC9050.038.9349717
TGCGTTC8600.038.06455617
TACGTAC1800.037.88833617
CCAAGTT59100.037.77293817
AGCGTAC1100.037.19945517
TTCGTAC2200.037.19945517
CTCGTTC6450.037.0072117
AGCGTTC8000.036.94112817
GCCGTTC9400.036.51790617
GACGTTC8500.035.57045717
Produced by FastQC (version 0.11.5)