FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRi-T0_S4_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRi-T0_S4_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6548306
Sequences flagged as poor quality0
Sequence length18-23
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAT208.5860695E-443.21551517
CGCGTTC551.8189894E-1243.21551517
TACGTTC1300.041.5533817
ATCGTTC1600.040.51454517
CCAACTT46700.036.59900717
GTTCGAT301.4468134E-436.01292817
CCATCTT21400.035.74367517
GTGCGAT554.087269E-935.3581517
GTCGTTC2850.034.8756817
TGGCGAT751.8189894E-1234.5724117
GAGCGAT451.1945012E-633.6120717
AGGCGAT1050.032.92610517
CCAAGTT37250.032.71617517
GACCGAT609.553332E-932.41163317
CCAACAT51450.032.25414317
CTCGTTC3400.031.14058917
GCCGTTC4750.030.9332117
TTTCGAT1050.030.86822317
TGTCGAT1400.030.86822317
TGCGTTC4200.030.35375417
Produced by FastQC (version 0.11.5)