FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRi-T0_S4_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRi-T0_S4_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6793199
Sequences flagged as poor quality0
Sequence length18-23
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTC1650.041.64721717
CGCCGAT200.001029936841.64721717
GTTCGAT256.146549E-541.64721317
ATCGTTC4750.040.3320417
TACGTTC3800.040.00324617
GTCGTTC7700.038.94285217
GGCGTTC7900.037.95695517
CCATCTT49600.037.86873617
GACGTAC1000.037.48249417
ATCGTAC351.06319985E-535.69761317
CACGTTC9300.035.37774317
GACGCTT1250.034.9836617
TTCGTTC6800.034.91016817
GCCGTTC11250.034.42836417
AGCGTTC7150.034.36623417
CTCGTTC7950.034.31311417
TCCGTTC7300.034.23058717
GACGTTC7700.034.07499717
AACGTTC6200.033.9223317
GTCGCTT2250.033.31777217
Produced by FastQC (version 0.11.5)