FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRi-T0_S4_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRi-T0_S4_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6800065
Sequences flagged as poor quality0
Sequence length15-23
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAT204.981489E-448.2641517
TACCGAT150.00969541948.2641517
GTTCGAT307.5489326E-540.22012717
CCAACTT27900.040.13363317
CAAAAGG250.001494267338.6113217
ATCGTTC1000.038.6113217
GTCGTTC1550.037.3657917
CCAAGTT31450.035.9103717
TACGTTC703.8380676E-1034.47439617
CCAACAT46700.033.84691617
GGTCGTT1250.032.81962217
AACAACG3250.032.6711217
CGCCGAT404.0938763E-430.16509417
ACCATCT22900.030.1387517
CTCGTTC2150.029.18297817
AACAAGG29900.029.05534217
TGTCGAT1250.028.95849217
TCACGAT752.859997E-828.9584917
GGCGTTC2250.028.9584917
GCCATCT20800.028.65684117
Produced by FastQC (version 0.11.5)