FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRi-T0_S4_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRi-T0_S4_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6583834
Sequences flagged as poor quality0
Sequence length15-23
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC900.044.01848617
CGTCGAT254.4333065E-544.01848617
TACGTTC3950.044.01848617
GACGTAC1200.042.18438317
TACGTAC1150.042.1046417
ATCGTTC5050.041.40352617
GTCGTTC5800.041.36219817
CGCGTTC1600.041.26733417
GAACGAT501.3442332E-939.6166417
GGCGTTC7550.039.35427517
CACGTTC8150.037.5372417
GACGTTC8100.037.4972317
TTCGTTC6000.037.41571417
GCCGTTC9550.037.33505217
CCAAGTT52500.037.31090517
AACGTTG601.9826984E-1036.68207617
TCCGTTC6500.036.56920217
AACGTTC5800.036.42909217
CTCGTTC6900.036.363117
TGCGTTC8100.035.3234817
Produced by FastQC (version 0.11.5)