FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRi-Rep2_S10_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRi-Rep2_S10_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6473241
Sequences flagged as poor quality0
Sequence length15-23
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGAT1000.039.22403717
GCTCGAT501.4806574E-939.22403717
TACGTTC1300.036.877317
ATCGTTC1850.036.51487417
GTGCGAT602.2009772E-1036.31855417
CCATCTT23150.036.24011617
CCAACTT42900.035.75980817
CGTCGAT250.002465986934.8658117
GTCGTTC2700.034.70439517
CCAAGTT34800.033.12502717
CCAACAT51150.031.27016817
GATCGAT353.4039762E-431.1301917
TCCGTTC2750.030.90378817
AGCGTTC2900.030.80815317
CGCGTTC651.91867E-830.17233817
GTACGTT651.91867E-830.17233817
TTCGTTC3550.030.077917
GTCGCTT1100.029.71518317
ACCCTAC6700.029.5969117
CACGTTC4000.029.41802817
Produced by FastQC (version 0.11.5)