FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRi-Rep2_S10_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRi-Rep2_S10_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6694666
Sequences flagged as poor quality0
Sequence length18-23
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAT200.001010038741.81264517
TACGTTC4400.041.337517
ATCGTTC5050.040.57068617
GTCGTTC6800.040.2754217
CGCGTTC1100.039.9120717
TACGTAC650.038.59628717
GACGTAC609.094947E-1238.3282617
GCTCGAT551.3460522E-1038.01149717
CCATCTT44350.037.71151717
GGCGTTC8750.037.03405817
GACGTTC7150.036.2571217
TTCGTTC7150.036.2571217
GCCGTTC10750.035.58936717
CTCGTTC6800.035.35628517
TGCGTTC9100.034.69071217
CACGTTC9200.034.08639517
GCCGCTT2150.034.0335517
AGCGTTC7500.033.45011517
TCCGTTC7150.033.33315717
AACGTTC6250.033.11561617
Produced by FastQC (version 0.11.5)