FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRi-Rep2_S10_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRi-Rep2_S10_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6687016
Sequences flagged as poor quality0
Sequence length18-23
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGTT403.7343852E-948.16970417
GAGCGAT150.00977071648.169717
TTTCGAT607.4578566E-1140.1414217
TACGTTC607.4578566E-1140.1414217
CCAACTT28500.037.69067817
ATCGTTC1100.037.22204617
CCAAGTT31850.035.54122517
GTCGTTC1100.035.03251317
GATCGAT657.23594E-933.3482617
TGGCGAT804.5474735E-1133.11667317
CCAACAT40450.032.45054217
ACTCGTT452.1936667E-532.11313617
CGGCGTT300.00368995932.11313217
TCCGTTC2000.031.31030717
TCACGAT552.4521796E-630.65344817
GATCGTT552.4521796E-630.65344817
AACAAGG27700.030.60602417
ACCATCT23500.030.13168517
TACCGAT404.1411057E-430.10606617
AACAACG3500.028.90182517
Produced by FastQC (version 0.11.5)