FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRi-Rep2_S10_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRi-Rep2_S10_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6478535
Sequences flagged as poor quality0
Sequence length15-23
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAC1400.043.8256617
ATCGTAC1100.043.8256617
TACGTTC3100.042.4119317
GTCGTTC5550.041.85153217
ATCGTTC4400.040.83754717
CGCGTTC1350.040.57931517
TACGTAC950.039.21243317
GGCGTTC7950.038.8642717
TTCGATC850.038.66969717
TTCGTAC1250.038.56657817
GGACGAT700.037.56485417
GCCGTTC9400.037.2984417
CGCCGAT651.2732926E-1137.08325617
ACCGTTC5500.037.05260517
CTCGTTC6650.036.9058217
CACGTTC7550.036.85999317
CCAAGTT49250.036.75126617
GACGTTC7200.036.2170417
GTCGTTG553.5634002E-935.85735717
AGCGTTC6750.035.709817
Produced by FastQC (version 0.11.5)