FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRi-Rep1_S7_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRi-Rep1_S7_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6409947
Sequences flagged as poor quality0
Sequence length18-23
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAT302.866671E-643.2054317
CGTCGAT254.9489543E-543.2054317
CGCGTTC600.043.2054317
TACGTTC1800.042.0052817
GACGTAC301.4487916E-436.00452417
ATCGTTC1950.035.4506117
GTCGTTC2400.035.10441217
GCTCGAT800.035.10441217
CCAACTT44350.034.58382817
CCAAGTT35150.033.740817
GTTCGAT451.1966677E-633.60422517
GGCGTTC3700.033.27985817
CCATCTT20350.033.12062517
TGTCGAT1750.032.09546317
CTCGTTC3800.031.8355817
CCAACAT50300.031.05121817
CGCCGAT353.581749E-430.86102317
AGACGAT502.7234673E-630.24380117
ACCCTAC6200.029.61662517
TCCGTTC3150.029.48942217
Produced by FastQC (version 0.11.5)