FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRi-Rep1_S7_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRi-Rep1_S7_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6657448
Sequences flagged as poor quality0
Sequence length15-23
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAT200.001035113841.60476717
TACGTTC3700.039.3558617
ATCGTTC5100.038.74953517
ATCGTAC700.038.63317
GTCGTTC7200.037.2709417
CCATCTT45400.037.25184617
GTTCGAT406.398859E-736.4041717
GTGCGAT406.398859E-736.4041717
GGCGTTC8300.036.0908817
TTCGTTC6800.035.4864217
TCCGTTC7650.035.35045617
GACGTTC7200.035.24848617
AGCGTTC8150.035.22366717
GCCGCTT3050.034.78431717
CGCGTTC1200.034.6706417
CACGTTC10150.034.63648217
CTCGTTC7500.034.1159117
GCTCGAT555.9226295E-934.0402617
AACGTTC6250.033.6166517
GCCGTTC11350.033.5404117
Produced by FastQC (version 0.11.5)