FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRi-Rep1_S7_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRi-Rep1_S7_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6622633
Sequences flagged as poor quality0
Sequence length15-23
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGAT950.040.29211417
TACGTTC551.5097612E-939.1474517
CCAACTT28550.037.79149617
ATCGTTC1200.035.88516217
CGCGTTC556.582923E-834.79773317
CCAAGTT28500.034.7519517
GGTCGTT704.2200554E-1034.17634617
TGGCGAT704.2200554E-1034.17634617
GTACGTT501.2280798E-633.49281717
CCAACAT42150.033.37362317
GAGCGAT300.003813463731.8979217
TACCGAT300.003813463731.8979217
AACAACG4850.031.0758117
AGGCGAT851.0004442E-1030.9597517
GGCGTTC2350.030.54056417
GACCGAT552.584171E-630.44801717
GAACGTT1100.030.44801717
ACCATCT22650.028.94049617
AACAAGG28550.028.57405917
AGTCGAT1350.028.3537117
Produced by FastQC (version 0.11.5)