FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRi-Rep1_S7_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRi-Rep1_S7_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6445285
Sequences flagged as poor quality0
Sequence length15-23
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAT351.5274418E-743.69800617
CGCCGAT254.628685E-543.69817
TACGTTC3450.043.064717
ATCGTTC4500.042.7269417
GAGCGAT650.040.3366217
GTCGTTC6450.040.31056217
AACGTTC5100.039.41388717
GACGTAC1450.039.1775217
TACGTAC1300.038.6559317
GGCGTTC7300.037.7119817
AGCGTTC7250.037.67069617
CTCGTTC6750.037.54791317
AGCGTAC2000.037.14330317
ATCGTAC651.2732926E-1136.97523517
CACGTTC9100.036.97523517
CCAAGTT48750.036.75114417
CTCGTAC2200.036.7460517
CGCGTTC1550.036.6499417
TTCGTTC5850.036.60174617
TTCGTAC1800.036.41500517
Produced by FastQC (version 0.11.5)