FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRa-T0_S5_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRa-T0_S5_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7395251
Sequences flagged as poor quality0
Sequence length16-23
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAT255.2081174E-542.83360717
TACGTTC1700.040.31398417
ATCGTTC2200.038.93964417
CGCCGAT405.0946255E-737.47940417
GTCGTTC4050.035.43026717
CCAACTT48600.035.16586317
GACGCTT950.033.81600617
CCATCTT27350.033.43683617
GAGCGAT451.2803848E-633.31502517
TACCGAT451.2803848E-633.31502517
TTTCGAT1400.032.12520617
AGACGAT1000.032.12520617
GTCGCTT1200.032.12520217
CCAACAT59550.030.9293917
CCAAGTT41050.030.6774217
TATCGAT701.382432E-930.59543417
TGCGTTC4900.030.15835617
AGCGTTC4500.029.98352617
GATCGAT1000.029.98352417
ATGCGAT502.9134771E-629.98352417
Produced by FastQC (version 0.11.5)