FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRa-T0_S5_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRa-T0_S5_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7660599
Sequences flagged as poor quality0
Sequence length16-23
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTTC4250.040.99444217
ATCGTTC5750.040.7610417
GTCGTTC8150.040.21000717
CGCGTTC1350.039.94608717
GACGTAC502.402885E-937.33422517
CCATCTT55750.037.12960417
GCCGCTT2750.036.2028917
ATCGTAC351.0925218E-535.55640417
GACGCTT1700.035.3821117
CTCGTTC7700.035.2870417
ACCGTTC6100.035.0220917
AGCGTTC8350.034.77572617
GGCGTTC10750.034.72951517
CGTCGAT301.840484E-434.56872617
CACGTTC11750.034.42162717
GCCGTTC14050.034.396517
TACGTAC850.034.16203717
AGCGTAC1100.033.9402117
TGCGTTC10650.033.88709317
CGCGTTG250.003141504733.18597817
Produced by FastQC (version 0.11.5)