FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRa-T0_S5_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRa-T0_S5_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7633408
Sequences flagged as poor quality0
Sequence length18-23
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTC1000.040.6498217
GATCGTT502.8572686E-838.25865617
GAGCGAT401.0291533E-535.8674917
TACGTTC801.8189894E-1235.8674917
GGTCGTT950.035.23823517
AACAACG4550.035.21057517
GGACGAT752.3646862E-1135.0704317
GTCGTTC1850.034.89809817
CCAACTT31450.034.67000217
CCAAGTT34350.034.59707317
CCAACAT48500.033.67353417
GCTCGAT501.2329147E-633.47632217
GTTCGAT452.3047434E-531.88221217
GTCCGAT452.3047434E-531.88221217
ACGCGTT452.3047434E-531.88221217
CGCCGAT300.003822780231.8822117
GGCGTTC3000.031.08515517
AACAAGG33550.030.14793417
GTACGTT404.3200955E-429.88957217
AGTCGAT1850.029.72800817
Produced by FastQC (version 0.11.5)