FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRa-T0_S5_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRa-T0_S5_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7430527
Sequences flagged as poor quality0
Sequence length15-23
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC1050.043.8650117
GACGTAC1600.042.4942317
TACGTAC1200.042.037317
GAGCGAT850.041.28471417
ATCGTTC5050.041.25916717
GTCGTTC6650.041.2265117
CGCGTTC1500.040.94067817
GGCGTTC8900.040.16851817
TACGTTC3700.039.71561417
CGTCGAT404.2279498E-738.3818817
CTCGTTC8050.037.87103317
CCAAGTT53100.037.0085217
CACGTTC11600.036.11300317
GACGTTC7800.035.99180217
AGCGTTC7100.035.8333917
TCCGTTC6550.035.8286717
ACCGTTC6350.035.57555817
TTCGTTC6150.035.3059817
TTCGTAC1750.035.09200717
AACGATC506.113805E-835.09200717
Produced by FastQC (version 0.11.5)