FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRa-Rep2_S11_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRa-Rep2_S11_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4716864
Sequences flagged as poor quality0
Sequence length16-23
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGAT208.268563E-443.54684417
TACGTTC1000.041.369517
CGCGTTC357.829187E-637.32586317
GTTCGAT301.3830978E-436.28903217
CTACGAT301.3830978E-436.28903217
CCAACTT30500.036.0510717
GTCGTTC1800.035.079417
CCATCTT15750.034.56098617
GGCGTTC2250.033.86976617
GAGCGAT451.1250395E-633.86976617
GGACGAT451.1250395E-633.86976617
AACGCTT850.033.3005217
AAACGAT401.954144E-532.66013317
GTGCGAT401.954144E-532.66013317
CACGTTC2850.032.08714717
CCAAGTT27350.031.92373517
ATCGTTC1200.030.84567617
CCAACAT37700.030.72535517
TCCGTTC2250.029.99893617
GCCGCTT1600.029.93845417
Produced by FastQC (version 0.11.5)