FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRa-Rep2_S11_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRa-Rep2_S11_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4896207
Sequences flagged as poor quality0
Sequence length16-23
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTTC3100.041.71539717
TTTCGAT553.6379788E-1241.71539317
GACGTAC401.3320459E-841.71539317
GAGCGAT200.001021609541.71539317
ATCGTTC3700.040.58794817
CGCGTTC1150.039.9016817
CCATCTT35000.037.8418217
GGCGTTC5350.037.4268917
GTCGTTC4850.037.41483717
TTCGTTC3800.036.77541417
ATCGTAC406.2660365E-736.5009717
GACGCTT1250.035.04092817
GACGTTC6200.034.65069217
CACGTTC7800.034.4954217
AGCGTTC6250.034.37348617
TACGTAC555.7698344E-934.13077517
GCCGCTT2100.033.76960417
AACGTTC4700.033.7273417
TGCGTTC6150.033.57580617
TCCGTTC4600.033.55368417
Produced by FastQC (version 0.11.5)