FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRa-Rep2_S11_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRa-Rep2_S11_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4878547
Sequences flagged as poor quality0
Sequence length18-23
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAT252.6235775E-548.1054917
GCGCGTT205.062617E-448.10548817
GAGCGAT301.3679291E-648.10548817
TACGTTC650.048.10548817
TGTCGTT900.040.08790617
AGACGAT502.7119313E-838.48439417
TATCGTT250.001518517438.48439417
GTCGTTC950.037.9780217
GCTCGTT651.8007995E-1037.0042217
CCAACTT20750.036.9774717
CCAAGTT22650.035.2561217
TGGCGAT703.9835868E-1034.36106517
GGACGAT351.9064406E-434.36106517
TTCGATC351.9064406E-434.36106517
CCAACAT30700.034.00290317
AACAACG3650.032.28998617
GATCGAT300.003713851332.07032417
GGTCGTT859.276846E-1131.1270817
TTCGTTC859.276846E-1131.1270817
ACCCTAC3450.030.67596417
Produced by FastQC (version 0.11.5)