FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRa-Rep2_S11_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRa-Rep2_S11_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4739990
Sequences flagged as poor quality0
Sequence length15-23
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAC1200.043.85855517
CGTCGAT302.5851314E-643.85855517
ATCGTAC502.910383E-1143.8585517
GTTCGAT502.910383E-1143.8585517
TACGTAC454.947651E-1043.8585517
TACCGAT254.5290755E-543.8585517
GGACGAT408.556526E-943.85854717
TACGTTC2750.043.06112317
ATCGTTC2600.043.0151217
GACGTAC850.041.27863317
GTCGTTC5000.040.78845217
GAGCGAT650.040.48481817
CGCGTTC1000.039.47269417
GCCGCTT950.039.2418617
CACGTTC7050.037.94853217
GGCGTTC5900.037.9116317
ACTCGAT700.037.59304417
CCAAGTT36650.036.67843217
ATCGAAC301.3262642E-436.54879417
TCCGTTC5200.036.2676517
Produced by FastQC (version 0.11.5)