FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRa-Rep1_S8_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRa-Rep1_S8_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7391730
Sequences flagged as poor quality0
Sequence length16-23
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTC503.6379788E-1142.892717
GAGCGAT551.0186341E-1038.99336617
TACGTTC1650.038.99336217
GTCGTTC2450.037.64053317
ACTCGAT750.037.17367617
ATCGTTC2200.036.06886317
CCAACTT46400.035.9133917
AGACGAT751.8189894E-1234.31416317
GGACGAT507.445851E-834.3141617
GTGCGAT507.445851E-834.3141617
ACGCGTT250.002666668534.3141617
GCCGCTT1100.033.14436317
CCATCTT25350.033.07898717
TTTCGAT850.032.800317
GGCGTTC3950.032.57673617
CCAAGTT41600.032.01486617
AGGCGAT1550.031.82361817
GTTCGAT551.719327E-731.19469517
CCAACAT59250.030.2239717
TTCGTTC4300.029.4263917
Produced by FastQC (version 0.11.5)