FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRa-Rep1_S8_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRa-Rep1_S8_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7655759
Sequences flagged as poor quality0
Sequence length15-23
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT200.001045707641.51895517
ATCGTAC303.7697973E-641.51895517
TACGTAC505.2750693E-1141.5189517
CGCGTTC1950.040.45436517
ATCGTTC5250.040.332717
TACGTTC3900.039.9220717
GACGTAC700.038.55331817
GTCGTTC9500.038.24114617
GGCGTTC9400.037.7645817
TGCGTTC9250.037.25484517
TTTCGAT850.036.63437317
CCATCTT52500.036.14126217
GTCGCTT1400.035.58767717
GGCGCTT2750.035.47983617
AGCGTAC1600.035.0316217
AACGTTC6500.034.81204617
AGACGAT1200.034.5991317
CGTCGAT301.8310008E-434.5991317
TCCGTTC7100.034.50166717
GACGTTC9850.034.3532517
Produced by FastQC (version 0.11.5)