FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRa-Rep1_S8_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRa-Rep1_S8_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7631577
Sequences flagged as poor quality0
Sequence length15-23
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAT205.20137E-447.8434517
TACGTTC553.092282E-1143.4940417
TACCGAT402.1395681E-741.86301817
GTCGTTC1950.040.48291817
GAGCGAT250.00155998838.27475417
CCAACTT31250.038.0451117
TGTCGAT900.037.2115717
ACGCGTT401.0261889E-535.88258717
ATCGTTC1200.035.8825817
GCTCGAT752.3646862E-1135.08519417
CCAAGTT38150.034.36200317
CGCGTTC351.9689242E-434.1738917
AATCGTT1400.034.1738917
CCAACAT50800.032.96317
TTTCGAT452.2981225E-531.8956317
ACTCGTT905.456968E-1231.8956317
AGGCGAT1250.030.61980217
GAACGAT552.5858208E-630.44582717
AACAACG4050.030.12365317
AACAAGG32300.029.84660517
Produced by FastQC (version 0.11.5)