FastQCFastQC Report
Wed 3 Aug 2016
Anshul1-CRISPRa-Rep1_S8_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnshul1-CRISPRa-Rep1_S8_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7436443
Sequences flagged as poor quality0
Sequence length15-23
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAT208.186602E-443.63591817
CGCGTTC1300.043.63591817
TACCGAT302.6778125E-643.63591817
GACGTAC1350.043.63591417
ATCGTTC4500.041.69654517
TACGTTC3150.041.55801417
ATCGTAC900.041.211717
GTCGTTC7900.041.15032617
TTCGTAC2450.040.96433317
GTGCGAT558.54925E-1139.66901817
GCCGATC1300.038.60117
TTTCGAT1250.038.3996117
GGCGTTC8250.038.34671417
TTCGTTC7600.037.6072717
TACGTAC1000.037.0905317
CCAAGTT55750.036.08279417
GACGTTC8650.036.06899617
AACGTTC5950.035.56877517
AGCGTTC7550.035.54448717
GAGCGAT800.035.4541817
Produced by FastQC (version 0.11.5)