FastQCFastQC Report
Mon 3 Dec 2018
RNA_48h_r3-ACAGTGAT_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA_48h_r3-ACAGTGAT_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122790
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19020.16939944246029978No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC1055.459002E-420.0040472
CGGAGAG4750.017.687791
CGCGGAA1350.002947821715.55454733
CGGCGAT1750.001016363914.0028331
CTCCGAT1500.00592716814.0028321
CTCGTAG1800.00125731813.6138651
CGGAGAT4002.25009E-913.1276561
CCGGGAT4509.749783E-1012.4469621
CGGTGAT2601.1741044E-412.1178371
CGGGGAT3802.1494998E-711.9761071
CTTACGG2050.003345729811.95150842
CGGGATT3601.4558227E-611.6690271
GTCGGGT3003.4259945E-511.6690271
GCGAGTC2459.6940424E-411.4308842
CCCGAGT4601.7929779E-811.4153531
CCCGTGT3702.024628E-611.3536481
CGGAAAG3752.379049E-611.2022671
CGGGGAG8150.011.1679041
CCCCGAT2550.001354523610.98261451
CGGGACT3206.614618E-510.9397131
Produced by FastQC (version 0.11.4)