FastQCFastQC Report
Mon 3 Dec 2018
Lib5-TCCTGMGC-GTGTAGAT_R2_001.trim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLib5-TCCTGMGC-GTGTAGAT_R2_001.trim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9006161
Sequences flagged as poor quality0
Sequence length19-76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACC1023431.1363665384174235No Hit
CCCTAACCCTAACCCTAACC620150.6885841814286909No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACC384640.4270854140848692No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC227100.25216071531477174No Hit
CCCTAACCCTAACCCTACCC160140.17781161140690246No Hit
CACACACACACACACACACA104850.11642030383423083No Hit
CCCTAACCCTAACCCTAACCCTAAC103700.1151434001679517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAGT2500.074.7554469
TAGATCA4250.069.5219770
GTCGCAG2800.065.4002268
TGATCGA706.4064807E-965.342966
CGCAGTA2950.064.3877970
TAACAGG554.8585844E-663.95419770
GTCGCCA9400.062.77184768
ATGATCG1650.062.45411365
AGGTCAC451.6610217E-460.28662568
GGTCGCA3450.057.91581767
TGATCGC5800.056.33009366
GTGATCG4950.055.94847565
CGCCGTA34350.055.7062570
GTGGTCA2450.055.2049865
CAATGGT551.1663542E-555.17135261
TGGTCAC2850.055.02560866
TAGTCAC2400.054.4524266
GCCGTAT19700.053.7581469
TGGTCGC42600.053.3787166
GTAAATG400.006817504352.76221570
Produced by FastQC (version 0.11.4)