FastQCFastQC Report
Mon 3 Dec 2018
Lib5-TCCTGMGC-GTGTAGAT_R1_001.trim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLib5-TCCTGMGC-GTGTAGAT_R1_001.trim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9006161
Sequences flagged as poor quality0
Sequence length19-76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGGGTTAGGGTTAGGGTTAGG1343671.491945347190662No Hit
GGGTTAGGGTTAGGGTTAGG1052931.1691218933350183No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG599630.6657997786182148No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG377770.4194573026176192No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA317680.35273631017699997No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA296450.3291635581464733No Hit
GTGTGTGTGTGTGTGTGTGT268040.2976184858343083No Hit
GAGAGAGAGAGAGAGAGAGA162650.1805985924524334No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA151550.16827369619530452No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG131040.14550039689497002No Hit
GGTTAGGGTTAGGGTTAGGGTTAGG111520.12382634509864969No Hit
GAAAGAAAGAAAGAAAGAAA103720.11516560718823482No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGA100930.11206772785874025No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA97350.10809267122806265No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGA94590.10502810242899278No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGA92980.10324043729620203No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTCC151.386877E-4140.702770
TGCCGTG151.386877E-4140.702770
CAGTCAT100.007758945140.7026870
CCAACTT100.007758945140.7026870
TCAACTT100.007758945140.7026870
AACGTCA100.008144332138.439469
TTCAGCT100.008144332138.439469
CTTCAGC100.008655571135.6482468
CTCACGG100.009137844133.2096467
ACCATAT650.0117.1410369
ACCTAAT257.5102307E-6112.5621570
TTCCATA257.5102307E-6112.5621570
TACAGTA257.5102307E-6112.5621570
TTACATC259.007788E-6108.51858568
TGCCGTA2950.0107.3156170
AATCCAT259.857524E-6106.5677167
AACATCC204.6487467E-4103.8295569
ACCGTAT204.6487467E-4103.8295569
TACCGTA950.0103.6756670
ATCCATA205.0409517E-4101.7361868
Produced by FastQC (version 0.11.4)