FastQCFastQC Report
Mon 3 Dec 2018
Lib0-TAAGGCGM-GTGTAGAT_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLib0-TAAGGCGM-GTGTAGAT_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10870322
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCCTGTCTCTTATACACATCTGACG490530.4512561817396026No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCCTGTCTCTTATACACAT490190.45094340351647355No Hit
CTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCT436360.40142325130755097No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCCTGTCTCTTAT387410.35639238653647975No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCCTGTC127270.11708024840478506No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATGG7500.050.86527670
GGCCCTA38100.049.054685
ATGCGCT1500.048.999794
ATATGCG2550.045.2976722
TCAATAG14650.044.67454570
GCCCTAG14100.044.1840026
GTCACCG6250.043.67880670
TCGATGG20600.043.66385370
GTAAGCT1800.042.77837434
ACATGCG5200.041.0609132
CAGGATT350.001256150540.00056534
TCAGTGG17600.039.7716470
GAACGCT8700.039.42584234
TAGATCA17350.039.13437370
GGTCACC6400.038.28020569
GTTAGCA556.7037854E-638.18235834
TGACCGA1300.037.6924929
TAGCCCT62000.036.6933944
CAGTACG1051.4551915E-1136.6675232
GCCCTAA72450.036.5697256
Produced by FastQC (version 0.11.4)