FastQCFastQC Report
Mon 3 Dec 2018
Lib0-TAAGGCGM-GTGTAGAT_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLib0-TAAGGCGM-GTGTAGAT_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10870322
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA878920.8085501055074542No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGCTGTCTCTTATACACAT790700.7273933559649842No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGCTGTCTCTTAT790010.7267586001592224No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA494450.4548623306650898No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGCTGTCTCTTATACACATTTCCGA355930.32743280281853654No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGCTGTCTCTTATACACATCTCCGA355760.3272764137069721No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGCTGTC338870.31173869550506417No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG216100.19879815887698635No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA177710.16348181774192153No Hit
GGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGG149640.13765921561477204No Hit
GGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAA112730.10370437968626872No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA110920.10203929561608202No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTCC150.002225386270.0002068
GCCTTAA150.002225426769.99988616
CTTCCAG150.002225426769.99988610
CAGCCTT150.002225426769.99988614
TCTTCCA150.002225426769.9998869
CCGGCAT150.002225426769.99988649
TTCCAGC150.002225426769.99988611
CCCCATG150.002225426769.99988643
TACTGCA453.8562575E-1062.2221235
GTAGTCC401.0682925E-861.24989734
TACCGTA1400.059.99989770
CCTGAAA308.3849245E-658.33323748
ACGTTGC252.3919607E-455.99990541
CCGTGTT252.3919607E-455.99990549
TGCCATA1400.054.99990570
GTACGTC200.00695353152.5001533
CTGTCCT200.006953657652.49991262
CCTTAAG200.006953657652.49991217
CCGGGAT200.006953657652.49991247
CTACTGC200.006953657652.49991234
Produced by FastQC (version 0.11.4)