FastQCFastQC Report
Mon 3 Dec 2018
Lib0-TAAGGCGM-GTGTAGAT_R1_001.trim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLib0-TAAGGCGM-GTGTAGAT_R1_001.trim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10870322
Sequences flagged as poor quality0
Sequence length19-76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGGGTTAGGGTTAGGGTTAGG3208062.9512097249741083No Hit
GGGTTAGGGTTAGGGTTAGG2542082.3385507807404418No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG1426531.3123162312947123No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG927390.8531394010223433No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG357990.3293278708763181No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA289670.26647784674639813No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA263510.24241232228447324No Hit
GTGTGTGTGTGTGTGTGTGT260700.2398273022639072No Hit
GGTTAGGGTTAGGGTTAGGGTTAGG241060.22175976019845595No Hit
GGGTTAGGGTTAGGGTTAGGG205740.18926762243105585No Hit
GGTTAGGGTTAGGGTTAGG185400.17055612520033905No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGG180430.16598404352695348No Hit
GAGAGAGAGAGAGAGAGAGA161920.14895602908543096No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG150850.1387723381147311No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA144710.13312393137940165No Hit
GTTAGGGTTAGGGTTAGGGTTAGG111450.1025268616697831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA1300.0113.6695270
CTGTCCT153.6389352E-4110.424362
TGCCATA1400.096.7544270
GTCAACG156.1905826E-496.613852
CCGGCAT157.465188E-492.16843449
ATACCGT2200.090.9833769
ACTCTCG207.859005E-490.9833669
CATTAAT208.4031676E-489.4632568
TACCATA3350.088.22111570
TTCCATA357.3116826E-787.95856570
TGCCGTA4250.086.9237570
TCCTCTT209.925336E-485.7916265
CCATCTT2150.077.3217170
TACAGTA401.617891E-676.9637470
CTCATCA200.001558389376.5769256
CCATCAT507.050221E-873.88518570
CCGTGTT256.130859E-573.7347549
CCGGATA256.711896E-572.39944548
GCCATCT1100.071.6838769
CGTCAAC200.0020422271.5322751
Produced by FastQC (version 0.11.4)